University Diagnostic Laboratory
Our mission is to protect and promote animal and human health through early detection and monitoring of animal diseases.

The University Diagnostic Lab (UDL) of the University of Veterinary & Animal Sciences, (UVAS) Lahore is a national leader in providing rapid diagnosis of animal diseases, identifying emerging diseases, developing new diagnostic methods and training diagnosticians, and veterinarians. Recently, UDL has merged with newly formed Institute of Microbiology, UVAS. UDL serves the interests of the livestock and poultry industry, animal research scientists and educators, and companion animal owners.
In 2009, UDL became the Pakistan's first ISO 17025 accredited veterinary disease diagnostic lab that provides ISO-certified diagnostic tests for the identification and monitoring emerging diseases. UDL faculty also works on developing new diagnostic methods. The lab obtained its accreditation from Pakistan National Accreditation Council (PNAC), which has mutual recognition arrangements (MRAs) with International Laboratory Accreditation Cooperation (ILAC) and Asia Pacific Laboratory Accreditation Cooperation (APLAC). It is a milestone in the history of UVAS and livestock sector of Pakistan that a veterinary disease diagnostic laboratory's results are now acceptable worldwide. The lab has also implemented a computer based "Diagnostic Information Management System (DIMS)" to ensure the good lab practices (GLP) and confidentiality of the client results. UDL provides quality diagnostic services for all animal species, including necropsy of birds, bacteriology, clinical chemistry, serology, virology, parasitology, molecular diagnostics, and toxicology.

Staff Position

Coordinator/ Professor

Associate Professor 

Assistant Professor 

  1. Dr. Fareeha Akhtar
  2. Dr. Rabia Tanvir
  3. Dr. Muhammad Suleman

Publications:

Complete Genome Sequences of 16 Mycoplasma bovis Isolates from Canadian Bison and Cattle. Karen B Register, Darrell O Bayles, Hao Ma, M Claire Windeyer, Jose Perez-Casal, Ana L Bras, Muhammad Suleman, Murray Woodbury, Murray D Jelinski, David P Alt. Microbiol Resour Announc. 2020 Jun 4;9(23):e00325-20.  doi: 10.1128/MRA.00325-20.

Effect of storage temperature on the microbiological quality of fish meat from two different managemental
systems. Ridda Haroon, Fareeha Akhtar, Ali Ahmad Sheikh. Accepted in Pakistan journal of zoology. 2020

Comparative antibiotic resistance profile of Acinetobacter spp. isolated from fish, chicken and beef meat
Aftab Ahmad, Fareeha Akhtar, Muhammad Ilyas Khan, Hameed Ur Rehman, Syeda Alia Gerdazi, Uzma Ghani, Muhammad Hanzalah Khan, Uzma Ayaz, Hira Mushtaq, Nausheen Abdul Malik, Nasir Mahmood, Matiullah,Wahid Shah, Malik Nawaz Shuja. 2019. Int. J. Biosci. 14(4), 305-316,

Study of the current resistance pattern to quinolones and other antimicrobials by multidrug resistant Escherichia coli recovered from retail chicken meat. F. Yasin, A. A. Sheikh, M. Rabbani, A. Aslam, M. I. Rasheed and R. Tanvir. The Journal of Animal & Plant Sciences, 29(6): 1580-1586.

Immune modulatory and anti-oxidative effect of selenium against pulmonary tuberculosis. Muhammad Imran Hussain, Waqas Ahmed, Muhammad Nasir, Muhammad Hassan Mushtaq, Ali Ahmad Sheikh, Arfat Yousaf Shaheen, Shahbaz Ali and Sannaullah Iqbal (2019). Pak. J. Pharm. Sci., Vol.32, No.2(Suppl), 779-784.

Immune boosting role of vitamin E against pulmonary tuberculosis. Muhammad Imran Hussain, Waqas Ahmed, Muhammad Nasir, Muhammad Hassan Mushtaq, Ali Ahmad Sheikh, Arfat Yousaf Shaheen and Ashraf Mahmood (2019). Pak. J. Pharm. Sci., Vol.32, No.1(Suppl), 269-276

Effect of carboxylated poly L-Lysine as a cryoprotectant on post-thaw quality and in vivo fertility of Nili Ravi buffalo (Bubalus bubalis) bull semen. Tariq, A., Ahmad, M., Iqbal, S., Riaz, M. I., Tahir, M. Z., Ghafoor, A., & Riaz, A. (2019).  Theriogenology.

Saleem N, Nawaz M, Ghafoor A, Javeed A, Mustafa A, Rizwan M. 2018. Phenotypic and Molecular Analysis of Antibiotic Resistance in Lactobacilli of Poultry Origin from Lahore, Pakistan. Pakistan Veterinary Journal. DOI: 10.29261/pakvetj/2018.084.

Ali M, Yaqub T, Mukhtar N, Imran M, Ghafoor A, Shahid MF, Yaqub S, Smith G, Su YC, Naeem M. 2018. Prevalence and Phylogenentics of H9N2 in Backyard and Commercial Poultry in Pakistan. Avian Diseases. 62: 1-9.

Jibreel M, Ahmad A, Rabbani M, Mushtaq H, Ghafoor A, Shabbir MZ, Saleem MH, Saleemi MK, Avais M, Muhammad J, Rehman H. 2018. Evaluation of Drinking Water Quality at Zoo and Various Public Places in District Lahore, Pakistan. Journal of Animal and Plant Sciences. 28(5): 1314-1320.

Nasim F, Javeed A, Ashraf M, Ghafoor A. 2018. Evaluation of immunomodulatory activity of tenoxicam in mice. Tropical Journal of Pharmaceutical Research. 17(9):1811-6.

Ali M, Yaqub T, Mukhtar N, Imran M, Ghafoor A, Shahid M, et al. 2019. Avian Influenza A (H9N2) Virus in Poultry Worker, Pakistan, 2015. Emerg Infect Dis. 25(1):136-139. Doi.org/10.3201/eid2501.180618.

Safdar I, Sheikh AA, Akhtar F, Muhammad J , Hussain T, Gohar M, Kanwal A and Tabassum A. 2018. Optimization of multiplex PCR for detection of gram positive foodborne pathogens from retail quail meat. Pak. J. Agri. Sci. 55(4), 955-958.

Ahmad A, Akhtar F,Khan IM, Rehman UH, Gerdazi AS, Ghani U, Khan MH, Ayaz U , Mushtaq H, Malik AN , Mahmood N, Matiullah , Shah W, Shuja NM. 2019. Comparative antibiotic resistance profile of Acinetobacter spp. isolated from fish, chicken and beef meat. International Journal of Biosciences. 14(4): 305-316.

Tanvir R, Sheikh AA, Javeed A. 2018. Endophytic Actinomycetes in the Biosynthesis of Bioactive Metabolites: Chemical Diversity and the Role of Medicinal Plants. In Studies in Natural Products Chemistry. Elsevier. 60: 399-424.

Nawaz F, Tanvir R, Nawaz M, Javeed A, Sajid I. 2018. The effects of polar and non polar compounds from endophytic actinomycetes in Ocmium tenuiflorum (Tulsi) and Azadirachta indica (Neem) on veterinary and human pathogens. Proceedings of 4th International Electronic Conference on Medicinal Chemistry ECMC-4. Doi:10.3390/ecmc-4-05567

Laboratories Sections

  • Bacteriology/Mycology
  • Molecular Biology
  • Serology
  • Parasitology

Diagnostic Services

UDL offers a wide range of testing at affordable prices (see list below).

Scope of testing

The latest tests range offered by UDL include:

TEST OFFERED AT UDL

RATES
(Rs.)

POULTRY

Postmortem for poultry (For one shed)

200

ELISA for detection of antibodies against Infectious bronchitis

350

ELISA for detection of antibodies against IBD (Gumboro) virus

450

ELISA for detection of Antibodies against Mycoplasma gallisepticum

350

ELISA for detection of Antibodies against Mycoplasma synoviae

350

HI Test for antibodies against New Castle disease (ND) or Avian Influenza (AI) or Egg Drop Syndrome (EDS) viruses, (1-8 Samples)

350

Culture and sensitivity Test

500

Total viable count (water)

500

Total viable count (feed, feed ingredients, Hatchery fluff, etc.)

1000

Coliform count (water)

500

Coliform count (feed, feed ingredients, Hatchery fluff, etc.)

1000

Isolation and identification of Salmonella (Species)

2000

Isolation and identification of E. coli (Species)

1500

Isolation and identification of Staphylococcus (Species)

1500

Isolation and identification of Pasteurella multocida

1500

Isolation and identification of Mycoplasma gallisepticum

2000

Isolation and identification of clostridium spp

3500

Isolation and identification of bacteria from food/ commercial product

2500

Plate exposure for bacteria (Environmental testing) / swab testing (one plate)

1000

Antibacterial activity of disinfectant

5000

Antiviral activity of disinfectant

5000

Isolation and identification of Avian Influenza (AI) & New Castle disease (ND) Virus

3000

PCR for diagnosis of Bird flu (H5N1) virus

3500

PCR for diagnosis of Avian Influenza (H9N2) virus

3500

PCR for diagnosis of Newcastle Disease virus

3500

PCR for diagnosis of Mycoplasma gallisepticum

3000

PCR for diagnosis of Salmonella spp

3000

PCR for diagnosis of Infectious Bronchitis Virus

3500

PCR for diagnosis of Chicken Anemia Virus

3500

BOVINES

ELISA for detection of Antibodies against Foot & Mouth Disease Virus (FMDV),  (Specific for Cattle & Buffalo)

800

ELISA for detection of Antibodies against Infection bovine Rhinotracheitis (IBR)

800

ELISA for detection of Antibodies against Brucella abortus

700

ELISA for detection of Antibodies against Johne's disease

800

ELISA for detection of Antibodies against Bovine Tuberculosis (TB)

800

ELISA for detection of Bovine viral Diarrhea Virus (BVDV)

800

ELISA for detection of Bovine leucosis

800

Rose Bengal Plate test for brucellosis

250

Tuberculin Test (Local antigen) for detection of tuberculosis

350

PCR for diagnosis of Brucella abortus

3000

PCR for diagnosis of Babesia bovis

2000

PCR for diagnosis of Theileria annulata

2000

PCR for diagnosis of Anaplasma spp

2000

PCR for diagnosis of Listeria monocytogenes

5000

PCR for diagnosis of Pasteurella multocida

5000

PCR for diagnosis of Bovine Viral diarrhea virus (BVDV)

5000

PCR for diagnosis of Mycobacterium spp

5000

PCR for diagnosis of Mycobacterium tuberculosis

5000

PCR for diagnosis of Mycobacterium bovis

5000

PCR for diagnosis of Campylobacter jejuni

5000

PCR for diagnosis of Bovine ephemeral fever

5000

PCR for diagnosis of Leptospira spp

5000

 

EQUINES

Mallein test (Local antigen) for detection of Glander

500

PCR for diagnosis of Burkholderia mallei (Glander)

3000

DOGS AND CATS

HA test for Canine Parvovirus (CPV) per sample

300

Strip test  for Canine Parvovirus (CPV) in dog

1000

Hyperimmune serum to cure the Canine Parvovirus (CPV) infected dog

 

ELISA for detection of Antibodies against Rabies virus (For export documentation)

6000

MISCELLANEOUS TESTS (Per Sample)

Blood test for parasite (Haemoparasites)

150

Complete blood count (Haematological Analyzer) 3 part machine

400

Complete blood count (Haematological Analyzer) 4 part machine

500

White side / surf test for early detection of mastitis per sample

150

Fecal examination for parasites ova detection

100

Fecal examination for parasites Egg per gram (EPG)

650

Fecal examination for parasites (Floatation technique)

350

Fecal examination for parasites (Sedimentation technique)

350

Skin scrapping for detection of ectoparasites

150

Skin scrapping for detection of fungus

1000

pH of water

250

Serum Electrolytes

Total serum / plasma Sodium

200

Total serum / plasma Potassium

200

Total serum / plasma Calcium

200

Total serum / plasma Chloride

200

Total serum / plasma protein

200

Total serum / plasma Phosphorus

200

Liver Function Test (LFT)

 

ALP

 

800

ALT

AST

Total Bilirubin

Renal Function Test

Serum / plasma Creatinine

500

Blood Urea Nitrogen

  • Cost of additional services i.e. preparation of any product, visit of farm (TA/DA, Transportation charges, etc), etc., will be charged separately as per requirement.
  • Lab animals are also available on cost basis.

Scope of ISO 17025 testing

Sr. #

Materials/Products tested

Types of test/Properties measured

Range of measurement

Minimum detection limit

Uncertainty of Measurement

Standard

  1.  

Serum

ELISA for detection of antibodies against IBD

0.25-9.0
(CPC value)

0.15

0.05

Kit Method (Synbiotic, USA 2016)

  1.  

Serum

ELISA for detection of antibodies against MG

0.25-9.0
(CPC value)

0.15

0.05

Kit Method (Synbiotic, USA 2016)

  1.  

Serum

ELISA for detection of antibodies against MS

0.25-9.0
(CPC value)

0.15

0.05

Kit Method (Synbiotic, USA 2016)

  1.  

Serum

Haemagglutination Inhibition Test for Avian Influenza (AI) H5

3.0-1024
(HI Titre value)

2.0

0.24

OIE (Manual updated 2019)

  1.  

Serum

Haemagglutination Inhibition Test for Avian Influenza (AI) H7

3.0-1024
(HI Titre value)

2.0

0.24

OIE (Manual updated 2019)

  1.  

 

Haemagglutination Inhibition Test for Avian Influenza (AI) H9

3.0-1024
(HI Titre value)

2.0

0.24

OIE (Manual updated 2019)

  1.  

Serum

ELISA for detection of antibodies against Rabies

0.25-9.0
(CPC value)

0.15

0.05

Kit Method (Synbiotic, USA 2016)

  1.  

Serum

ELISA for Foot & Mouth Disease (FMD)

0.25-9.0
(CPC value)

0.15

0.05

Kit Method (Prionics, USA 2016)

  1.  

Serum

ELISA for detection of antibodies against Brucella

0.25-9.0
(CPC value)

0.15

0.05

Kit Method (IDEXX, USA 2016)

  1.  

Serum

Rose Bengal Test (RBT)

Qualitative test

Not applicable

Not applicable

OIE Manual Updated 2019

  1.  

Water

pH of water

3:00-10:00

2:00

0.156

AOAC /2016, 20th Edi, Method # 973.41

  1.  

Equine Species

Mallein Test

Qualitative test

Not applicable

Not applicable

OIE Manual Updated 2019

DOGS AND CATS

HA test for exam; to confirm the presence of Canine Parvovirus (CPV) per sample

150

HI test for determining antibody titer against Canine Parvovirus (CPV) in dog serum

200

Hyperimmune serum to cure the Canine Parvovirus (CPV) infected dog (64 HI units of antibodies/dose)

200

Hyperimmune Serum to Cure early diagnosed Cases of Canine Distemper Virus (CDV) infected dogs (1:32 AGPT units of antibodies per dose)       

200

ELISA for detection of Antibodies of Canine Parvovirus & Canine Distemper

1500

ELISA for detection of Rabies antibodies

600

MISCELLANEOUS TESTS (Per Sample)

Tuberculin test (imported antigen) for detection of Tuberculosis

300

Tuberculin test (local antigen) for detection of Tuberculosis

100

Johnin test  (Imported antigen) for detection of Johne’s disease

200

Mallein test for detection of Glanders in equines

100

Blood test for parasite (Haemoparasites)

100

Complete blood count (Haematological Analyzer)

200

White side / surf test for early detection of mastitis

30

Fecal examination for parasites ova detection

40

Skin scrapping for detection of ectoparasites

50

pH of water

50

Hardness of water

50

Sodium

100

Potassium

100

Calcium

100

Lithium

100

Total serum / plasma protein

200

Total serum / plasma Calcium

200

Total serum / plasma Magnesium

200

Total serum / plasma Phosphorus

200

MOLECULAR BIOLOGICAL TESTS

PCR for diagnosis of Bird flu (H5N1) virus

2500

PCR for diagnosis of Avian Influenza (H9N2) virus

2500

PCR for diagnosis of Avian Influenza (H7N3) virus

2500

PCR for diagnosis of ND virus

2500

PCR for diagnosis of Rabies virus

2500

PCR for diagnosis of Canine Distemper virus

2500

PCR for diagnosis of Canine Parvo virus

2000

PCR for diagnosis of Mycoplasma gallisepticum

2000

PCR for other DNA viruses 

2000

PCR for other RNA viruses

2500

New Diagnostic test added and rates revised

 

Serum Electrolytes

400

·        Total serum / plasma Sodium

125

·        Total serum / plasma Potassium

125

·        Total serum / plasma Calcium

125

·        Total serum / plasma Chloride

125

Liver Function Test (LFT)

500

·        Alkaline Phosphatase

·        ALT

·        AST

·        Billirubin

Renal Function Test

300

·         Serum / plasma Creatinine

150

·         Blood Urea Nitrogen

150

Scope of ISO 17025

Materials/Products tested

 Types of test/ Properties measured 

Range of measurement

Minimum detection limit

Uncertainty of Measurement (where applicable)

Standard specification/ Techniques/ equipment used

Serum

 

ELISA for RABIES 0.25-9.00
(CPV Value)
0.15 0.05 Kit Method
Synbiotics, USA

ELISA for IBD

0.25-9.00

(CPC value)

0.15

0.05

 

Kit method (Synbiotics, USA)

2008

 

ELISA for  MG

0.25-9.00

(CPC value)

0.15

0.05

Kit method (Synbiotics, USA)

2008

 

ELISA for MS

0.25-9.00

(CPC value)

0.15

0.05

Kit method (Synbiotics, USA)

2008

 

HI for AI (H5)

3.0 - 1024

( HI TiterValue)

2.0

0.1

 

OIE  Test method 2008, 5th Edition

 

HI for AI (H7)

3.0 - 1024

( HI TiterValue)

2.0

0.1

OIE  Test method 2008, 5th Edition

 

HI for AI (H9)

3.0 - 1024

( HI TiterValue)

2.0

0.1

OIE  Test method 2008, 5th Edition

 

Rose Bengal Test

Qualitative Test

Not applicable

Not applicable

OIE  Test method 2008, 5th Edition

 

Water

pH of Water

 

3.00 – 10.00

 

 

 

2.00

 

 

0.5

 

AOAC Test method 2004, 17th Edition Ch-11.

Animal (Equines)

Mallein Test

Qualitative Test

Not applicable

Not applicable

OIE  Test method 2008, 5th Edition

 

Teaching, Research & Development

UDL faculty is also involved in teaching, research & development. A number of research projects have been successfully completed and few are still in process.

S#

Title of Project

Source of funding

Amounts of funds (Rs) in Million

1.

Preparation of highly specific ELISA Kit on Recombinant outer membrane protein (OMP) of Pasteurella multocida B2 to detect Post-vaccination immune response

HEC, Pakistan
Under TDF

9.544

2.

Preparation of live genetically engineered avirulent vaccinal strains derived from local highly pathogenic isolates of P. multocida B:2, using gene deletion method and evaluation of the vaccine by inoculating in mice and calves

HEC, Pakistan
Under NRPU

6.714

3.

Hydrogen peroxide based product CareOx35 effect on the health of dairy cows

Dexon

0.9

4.

Vaccine Efficacy Testing

ICI

1.3

5.

Effect of Different antibiotics and steroidal Residues in Meat of Chicken and their effect on Humans in Punjab

National Industrial funds

 

6.

Epidemiology and Characterization of Bacterial Pathogens Associated with Epizootic Ulcerative Syndrome (EUS) and Motile Aeromonas Septicemia (MAS) from Pakistani Farmed Fish

National (PARC)

 

7.

Molecular Characterization of Bovine Pestivirus isolates from dairy animal of Pakistan and their use in diagnostic, biosecurity and biological development to control disease in Pakistan herds

National (HEC)

6.022

8.

Comparative transcriptome analysis of innate and adaptive immune cells during SARS-CoV-2 infection

International (Qatar)

5.5

Human Resource Development

UDL is playing a vital role in human resource development through organizing workshops, providing trainings, refresher courses and diploma courses for UVAS staff, students, farmers, lab and field professionals and personnel from sister institutions of the country.

International Linkages

Sr. No.

University / College

Country

Nature of Cooperation

1

Division of Lab Animal Medicine, David Geffen School of Medicine, University of California, Los Angeles, CA

USA

Faculty development in Lab Animal Facility Management

Department of Pathobiology, The University of Connecticut, Connecticut

USA

Collaborative Research & Faculty Development

Institute of Animal Health, Compton

UK

- do -

4

Animal Diagnostic Laboratory, PennState University

USA

- do -

Atlantic Veterinary College, University of Prince Edward Island

Canada

Faculty Development

Agriculture & Agri Food Cooperation (AAFC)

Canada

- do -

7

University of Qatar

Qatar

Collaborative research

8

Vaccine and Infectious Disease Organization- International Vaccine Center (VIDO-InterVac)

Canada

Collaborative research

Clients

  • K & Ns Poultry Farms, Raiwind, Lahore
  • Supreme Farms, Lahore-Sheikhupura Road
  • The Livestock Farm, Rakh Dera Chahl, Lahore
  • RVFC (Mona depot, Abbotabad, Sahiwal etc.)
  • The Governor House, Lahore
  • The Zoo, Lahore
  • The Livestock Farms of the Punjab
  • The Clinics of UVAS, Lahore.
  • The Pets Clinics, Lahore
  • The Camel Owners, Lahore
  • The Kennel Club, Lahore
  • National Feed Pvt. Ltd, Raiwind, Lahore
  • The Race Course Club, Lahore.
  • The Rangers Lahore
  • The Police Line, Lahore
  • Rana Nisar Stud Farm, Phool Nagar, Pattoki
  • Munir Poultry Farm, Gujranwala
  • Idara-e-Kissan
  • The Crystal Feed Industry, Multan
  • Noor Stud Farm, Sharif City, Raiwind Road
  • Other Poultry Farmers of Punjab province
  • Private Veterinary Clinics

 

Location

University Diagnostic Lab (UDL), University of Veterinary and Animal Sciences – UVAS, Syed Abdul Qadir Jillani (Out Fall) Road, 54000 Lahore – Pakistan

Regular Business Hours    

Monday – Friday

8: 00a.m. - 4:00 p.m.

Parking

Temporary parking for UDL clients on four wheels is available in front of UDL. For motor bikes, in the parking stand on main outfall road.