Dr. Wasim Shehzad

Professor
IBBt, UVAS, Lahore
E. mail: wasim.shehzad@uvas.edu.pk
Contact no: 03004213043

(HEC Approved Supervisor)

Biography

My research interests cover a broad range of areas by exploiting DNA from non-invasive sampling, such as from feces, urine, soil, water or from hairs. The quality and quantity of such a DNA is feeble as compared with the DNA extracted from the tissue samples. Such an approach can be applicable to many molecular basis of identification of species, without touching or even observing them, or even ideal for elusive and endangered species. With the advent of next generation sequencing, we can generate millions of sequences from a single run. Such an approach is ideal for meta-barcoding (amplifying multiple species from a single sample). During my PhD study from University of Grenoble, Grenoble France, I have applied these approaches to study the diet of endangered felids of South-Asia and have published my results in peer reviewed journals, as well as in international scientific conferences. I have also won international research grants during my PhD study.

Area of Interest

  • Metagenomic approaches using non-invasive sampling techniques.

 


 

 

1. Qurat Ul Ain, Afshan McCarthy, Asif Nadeem, Katthy Niakan, Wasim Shehzad, Tahir Yaqub, Fouladi Nashta and Maryam Javed (2026). Evaluation of PX458 lipofection and puromycin selection in bovine oviduct epithelial cells. The Journal of Animals and Plant Sciences 36 (5).

2. Muhammad Sheraz Yasin, Wasim Shehzad, Kamran Ashraf, Rahat Naseer and K. Hussain (2025). Anthelmintic efficacy and safety of alkaloid-rich fractions of Nicotiana tabacum against benzimidazole-resistant Haemonchus contortus in goats. Veterinary World 18(11): 3420-3432.

3. Muhammad Muddassir Ali, Furqan Awan, Muhammad Usman Jamil, Muhammad Ijaz, Asad Ullah and Wasim Shehzad (2025). Genotoxic and Oxidative Stress Assessment of Heavy Metal Contaminated Soil and Water in Human Blood and Bovine Milk Samples from the Different Industrial Zones of Punjab, Pakistan. Journal of Trace Elements in Medicine and Biology: https://doi.org/10.1016/j.jtemb.2025.127790.

4. Quratul Ain Nazir, Wasim Shehzad, Muhammad Asad Ali and M. Yasir Zahoor (2024). Mutation spectrum analysis of ITGA3 gene associated with nephrotic syndrome. Journal of Population Therapeutics & Clinical Pharmacology 31(5): 2024-2046.

5. Ullah U, Ashraf K, Shehzad W and R. MI. (2024). Designing a multi-epitope construct using immuno-informatic tools to prepare a messenger RNA vaccine against Rhipicephalus microplus ticks. Veterinary World 17(10):2235-2247.

6. Aslam, M., R. Seher, M. Z. Shabbir, W. Shehzad and M. Imran (2024). Direct PCR assays for DNA barcoding and sexing of plucked feathers. Gene Reports 36: 101937.

7. Qurrat-Ul-Ain, Imtiaz Rabbani, Muhammad Afzal Rashid, Muhammad Shahbaz Yousaf, Wasim Shehzad, H. Rehman. (2024). Effects of dietary garlic (Allium sativum) and papaya (Carica papaya) leaf powder on production performance, ruminal methanogen levels, gut parameters, and meat quality in goats. Veterinary World 17.

8. Sana Jafar, Salman Jamil, Muhammad Sheraz Yasin, Asif Naseem, Muhammad Yasir Zahoor, Wasim Shehzad, Muhammad Imran (2024). Successful application of modified crude DNA extraction from muscle tissues for various types of PCR amplifications. Molecular Biology Reports_ https://doi.org/10.1007/s11033-024-09356-1

9. Sattar S, Nadeem A, Shehzad W, ur Rehman H, Javed M. A biochemical and histological evaluation of in vivo exposure of bisphenol P for multi-organ toxicity and pathology in rats. Toxicology and Industrial Health. 2024;40(4):194-205. 

10. Shujat, S, W. Shehzad, A.A. Anjum, J.A. Herti, Y.T. Grohn, M.Y. Zahoor (2024). Molecular identification of Coxiella burnetii in raw milk samples collected from farm animals in districts Kasur and Lahore of Punjab, Pakistan. PLOS ONE 19(6): e0301611.

11. Sana Jafar, Khalid Mahmood Anjum, Muhammad Yasir Zahoor, Wasim Shehzad, Asif Naseem, Muhammad Imran (2024). Development and validation of a universal primer pair for the taxonomic and phylogenetic studies of vertebrates. Molecular Biology Reports. https://doi.org/10.1007/s11033-023-09175

12. Riaz Yasir, Ali Muhammad Muddassir, Khan Talha Jalil, Javed Maryam, Ijaz Muhammad, Wasim Shehzad (2024). Assessment of single nucleotide polymorphism RS1888747 in FRMD3 and RS6930576 in SASHI1 genes on diabetic nephropathy patients in Pakistani population. Genetika 56(1): 157-168.

13. Shujat, S, W Shehzad, AA Anjum, JA Hertl, MY Zahoor, YT Gröhn. 2023. Molecular detection of Coxiella burnetii in raw meat samples collected from different abattoirs in districts Kasur and Lahore of Punjab, Pakistan. PLOS ONE 18:e0289944.

14. Jafar S, Kabir F, Anjum KM, Zahoor MY, Shehzad W, Imran M. (2023). Comparison of different DNA preparatory methods for development of a universal direct PCR-RFLP workflow for identification of meat origin in food products. Food Science and Technology (Campinas). 43: DOI:10.1590/fst.65122.

15. Jafar S, Waheed F, Anjum KM, Shehzad W & Imran M. (2023). A low-cost closed-tube method for detection of adulteration in ground meat. Food Biotechnology. 37; 1, 25-40. DOI: 10.1080/08905436.2022.2163250

16. Aslam M, Naeem F, Seher R, Zubair Shabbir M, Shehzad W, Imran M. Effect of storage temperature and duration on direct PCR amplification of various feather types and DBS matrices (2023). Gene. 20; 854:147116. DOI: 10.1016/j.gene.2022.147116. Epub 2022 Dec 13. PMID: 36526120.

17. Bajwa, AA, W Shehzad, S Islam, M Imran, K Ashraf, A Khan, MY Zahoor, MI Rashid, WA Khan, HU Rehman, P Orozco-Terwengel. 2023. Demographic history of the Punjab urial and implications for its management. The Journal of Wildlife Management n/a:e22426.

18. Abdul Muqeet Khan, Muhammad Imran, Wasim Shehzad, Mateen Abbas (2023). Adsorbent filters for removing polycyclic aromatic hydrocarbons from the atmosphere. Environment Protection Engineering, 49 (2), DOI: 10.37190/epe230202

19. Abdul Muqeet Khan, Muhammad Imran, Abu Saeed Hashmi, Wasim Shehzad, Mateen Abbas (2022). Quantification of Selected Polycyclic Aromatic Hydrocarbons (PAHs) in Soil, Water and Blood by an Optimized and Validated HPLC –UV–DAD Method. Polish Journal of Environmental Studies, Vol. 31, No. 4 (2022), 1-12. https://DOI: 10.15244/pjoes/145994

20. Muhammad Usman Faryad Khan, Asif Nadeem, Maryam Javed, Wasim Shehzad & Muhammad Asad Ali (2022). Molecular characterization of methicillin-resistant Staphylococcus aureus (mrsa) isolated from bovine wounds, Jenetika, Vol. 54, No1, 411-426. https://doi.org/10.2298/GENSR2201411K

22. Ullah I, Murtaza K, Ammara H, Misbah, Bhinder MA, Riaz A, Shehzad W, Zahoor MY (2022). Association study of CLDN14 variations in patients with kidney stones. Open Life Sciences doi.org/10.1515/biol-2021-0134.

23. Rasool IG, Zahoor MY, Ahmed I, Iqbal M, Shafqat S, Anjum AA, Shehzad W. (2022). Description of novel variants in consanguineous Pakistani families affected with intellectual disability. Genes & Genomics doi: 10.1007/s13258-022-01219-y

24. K. H. Anjum, A. Nadeem, M. Javed, H. I. Ahmad, A. Riaz, W. Shehzad, J. Azhar, M. F. Bhutta (2022). Genomic and Computational Analysis of Novel SNPs in TNP1 Gene Promoter Region of Bos indicus Breeding Bulls. Genetics Research, 22. https://doi.org/10.1155/2022/9452234

25. Earnist S, Nawaz S, Ullah I, Bhinder MA, Imran M, Rasheed MA, Shehzad W, Zahoor MY (2022). Mitochondrial DNA diversity and maternal origins of Pakistani donkey. Brazilian Journal of Biology. 4;84.

26. A. Y. Shaheen, A. A. Sheikh, M. Rabbani, W. Shehzad, Z. Abbas, M. Maqbool (2021), isolation and physicochemical characterization of Brucella phages. Journal of Animal & Plant Sciences, 31(5) : 2021, Page: 1277-1286.

27. Sumera NS, SS Iqbal, ST Khan, ZU Rehman, W Shehzad (2021). Fusarium oxysporum silver nanoparticles; their characterization and larvicidal activity against Aedes mosquitoes. Intl J Agric Biol 26:115‒124.

28. Rasool IG, Zahoor MY, Iqbal M, Anjum AA, Ashraf F, Abbas HQ, Baig HM, Mahmood T, Shehzad W (2021). Whole exome sequencing revealed novel variants in consanguineous Pakistani families with intellectual disability. Genes & Genomics 43(5):503-512. https://doi.org/10.1007/s13258-021-01070-7.

29. Ullah I, Ottlewski I, Shehzad W, Riaz A, Ijaz S, Tufail A, Ammara H, Mane S, Shril S, Hildebrandt F, Zahoor MY, Mujmundar AJ (2021). Sequencing the CaSR locus in Pakistani stone formers reveals a novel loss-of-function variant atypically associated with nephrolithiasis.. BMC Medical Genomics 14(1):1-7.

31. Maryam Javed, Syed Ahmed Raza , Asif Nadeem, Muhammad Muddassir Ali, Wasim Shehzad, Khalid Mehmood. 2021. Exploring the Potential of Interferon Gamma Gene as Major Immune Responder for Bovine Tuberculosis in River Buffalo. BioMed Research International.

32. Arfat Yousaf Shaheen, Ali Ahmad Sheikh, Masood Rabbani, Wasim Shehzad, Zaigham Abbas, Munazzah Maqbool. 2021. Isolation, Propagation and Biocontrol Activity of Indigenous Bacteriophages against Brucella abortus. International Journal of Agriculture & Biology DOI: 10.17957/IJAB/15.1765.

33. Muhammad Ashraf, Ijaz Farid, Wasim Shehzad, Muhammad Rizwan Yousaf, Nasim Ahmad, Awais Ihsan, Khalid Javad, Hasan Riaz, A Riaz. 2021. Effects of Different Nutritional Regimens during Pre-Weaning Period on Body and Testis Growth, Sexual Behavior, Hormonal Profile and Genes Expression in Male Beetal Goat Kids. Pakistan veterinary journal DOI: 10.29261/pakvetj/2021.023.

34. Yousaf, R, I Khan, W Shehzad, R Hussain, S Ali, H Neubauer, G Wareth. 2021. Seroprevalence and Molecular Detection of Brucellosis in Hospitalized Patients in Lahore Hospitals, Pakistan. Infectious Disease Reports 13:166-172.

35. S. Suleman, W. A. Khan, K. M. Anjum, W. Shehzad, SGM Hashmi. 2021. GIS-based distribution and population estimation of Punjab urial (ovis vignei punjabiensis) in Pakistan. The Journal of Animal and Plant Sciences 31:https://doi.org/10.36899/JAPS.32021.36894.30317.

37. Zia Ur Rehman, Atsushi Tashibu, Michiyo Tashiro, Imran Rashid, Qasim Ali, Osama Zahid, Kamran Ashraf, Wasim Shehzad, Umer Chaudhry, Madoka Ichikawa-Seki. 2021. Molecular characterization and phylogenetic analyses of Fasciola gigantica of buffaloes and goats in Punjab, Pakistan. Parasitology International 82.

38. Zeshan Haider Raza, Asif Nadeem, Maryam Javed, Faiz-ul-Hassan, Wasim Shehzad, Arfan Ahmad, Muhammad Saadullah, MM Ali. 2020. Polymorphic status and phylogenetic analysis of Myostatin gene in Pak-thoroughbred. Genetika 52:1281-1290.

39. Islam, S, UK Reddy, P Natarajan, VL Abburi, AA Bajwa, M Imran, MY Zahoor, M Abdullah, AM Bukhari, S Iqbal, K Ashraf, A Nadeem, H Rehman, I Rashid, W Shehzad. 2020. Population demographic history and population structure for Pakistani Nili-Ravi breeding bulls based on SNP genotyping to identify genomic regions associated with male effects for milk yield and body weight. PLoS One 15.

40. Amna Arshad Bajwa, Saher Islam, Muhmmad Imran, Karman Ashraf, Arman Khan, Muhammad Farhan Khan, Imran Rashid, Muhammad Yasir Zahoor, Waseem Ahmad Khan, Wasim Shehzad (2020). DNA-Based Gender Identification of Punjab Urial (Ovis vignei punjabiensis) using Non Invasive Sampling. Pakistan Journal of Zoology, 52. DOI: http://dx.doi.org/10.17582/journal.pjz/2020.52.....

41. Kanwal Zahra, Muhammad Imran, Muhammad Yasir Zahoor, Kamran Ashraf, Kumail Hussain Jaffry, Asif Nadeem, Imran Rashid, Muhammad Younas, Maqsood Akhtar, Wasim Shehzad (2020). Molecular screening of Complex Vertebral Malformation and Citrullinemia carriers in Pakistani Nili-Ravi buffalo (bubalus bubalis) breeding bulls. The Journal of animal & Plant Sciences, 30(3).

42. Ghulam Abbas, Asif Nadeem, Maryam Javed, Muhammad Muddassir Ali, Muhammad Aqeel, Masroor Ellahi Babar, Muhammad Sajid Tahir, Sadia Tabassum, Wasim Shehzad (2020). Mitochondrial Cytochrome-b, Cytochrome-c and d-loop Region Based Phylogenetic and Diversity Analysis in Blackbuck (Antilope cervicapra). Kafkas Universitesi Veteriner Fakultesi Dergisi, DOI: 10.9775/kvfd.2019.22108

43. Javeria Zafar, Asif Nadeem, Maryam Javed1, Fehmeeda Fatima, Wasim Shehzad, Ghulam Abbas, Rajput Zahid Iqbal and Muhammad Muddassir Ali (2020). Assessment of Mitochondrial ATPase 8/6 Genes Based Genetic Diversity in Sindh Ibex (Capra aegagrus Blythi). Pakistan J. Zool., vol. 52(2), pp 441-446.

44. Iqbal, M, MS Saleem, M Imran, WA Khan, K Ashraf, M Yasir Zahoor, I Rashid, H-U Rehman, A Nadeem, S Ali, S Naz, W Shehzad. 2020. Single tube multiplex PCR assay for the identification of banned meat species. Food Additives & Contaminants: Part B:1-8.

45. M. Iqbal, W. Shehzad, M. Imran, M. Y. Zahoor, W. A. Khan, M. I. Rashid, K. Ashraf, H. Rehman, M. S. Saleem, A. Nadeem. 2020. DNA based gender identification of meat samples The Journal of Animal and Plant Sciences 30(6).

46. Zia Ur Rehman, Osama Zahid, Imran Rashid, Qasim Ali, Muhammad Haroon Akbar, Muhammad Oneeb, Wasim Shehzad, Kamran Ashraf, Neil D. Sargison, Umer Chaudhry (2020). Genetic diversity and multiplicity of infection in Fasciola gigantica isolates of Pakistani livestock. Parasitology International, 76.

47. Saher Suleman, Wasim Ahmed Khan, Khalid Mehmood Anjum, Wasim Shehzad & Hashmi, G. M. (2020). Habitat suitability index (HSI) model of Punjab Urial (Ovis vegnei punjabiensis) in Pakistan. The Journal of Animal & Plant Sciences, 30(1).

48. Huma Mujahid, Abu Saeed Hashmi, Muhammad Zargham Khan, Muhammad Tayyab, Wasim Shehzad 2019. Yeast sludge and its components ameliorate ochratoxin Ainduced toxicity in broiler chicks. Tropical Journal of Pharmaceutical Research 18 (10).

49. Huma Mujahid, Abu Saeed Hashmi, Muhammad Zargham Khan, Muhammad Tayyab, Wasim Shehzad 2019. Protective effect of yeast sludge and whey powder against Ochratoxicosis in broiler chicks Pakistan Veterinary Journal DOI: 10.29261/pakvetj/2019.077 1-5.

50. Bajwa AA, Cuff JP, Imran M, Islam S, Mansha R, Ashraf K, Khan A, Rashid MI, Zahoor MY, Khan WA, Rehman HU, Nadeem A, Orozco-terWengel P, Shehzad W 2019. Assessment of nematodes in Punjab Urial (Ovis vignei punjabiensis) population in Kalabagh Game Reserve: development of a DNA barcode approach. European Journal of Wildlife Research 65: 63. doi: 10.1007/s10344-019-1298-y

51. Kanwal Zahra, Muhammd Yasir Zahoor, Muhammad Imran, Kamran .Ashraf, Asif Nadeem, Imran Rashid, Habib-ur-Rehman, Kumail Hassan, Muhammd Younas, Maqsood Akhtar, Wasim Shehzad 2019. Molecular screening of FXI deficiency gene fragment in Nili-Ravi buffalo bulls of Punjab, Pakistan. The Journal of Animal & Plant Sciences 29: 899-904.

52. Habib M, Yaqub T, Aziz ul R, Sohail T, Shahbaz M, Shehzad W, Munir M, Shabbir MZ 2019. Evaluation of transmission potential and pathobiological characteristics of mallard originated Avian orthoavulavirus 1 (sub-genotype VII.2) in commercial broilers. Microbial Pathogenesis 137: 103785. doi: https://doi.org/10.1016/j.micpath.2019.103785

53. Saher Islam, Wasim Shehzad, Amna Arshad Bajwa, Muhammad Imran, Muhammad Yasir Zahoor, Muhammad Abdullah, Muhammad Imran Rashid, Kamran Ashraf, Yung-Fu Chang, Asif Nadeem, Muhammad Younas, Sayed Aamir Mehmood Bukhari, Muhammad Muzzammil Hassan, Zafar Iqbal Qureshi, Raheela Akhtar 2019. Molecular detection of Brucellosis, Leptospirosis and Campylobacteriosis by multiplex PCR and screening by ELISA Assays in buffalo breeding bulls Pakistan Veterinary Journal DOI: 10.29261/pakvetj/2019.092

54. Cabezas-Cruz A, Allain E, Ahmad AS, Saeed MA, Rashid I, Ashraf K, Yousfi L, Shehzad W, Indjein L, Rodriguez-Valle M, Estrada-Peña A, Obregón D, Jabbar A, Moutailler S 2019. Low genetic diversity of Ehrlichia canis associated with high co-infection rates in Rhipicephalus sanguineus (s.l.). Parasites & Vectors 12: 12. doi: 10.1186/s13071-018-3194-9

55. Abdullah Saghir Ahmad, Imran Rashid, Kamran Ashraf, Wasim Shehzad, Matiullah Khan, Kashif Hussain, Shahid Hussain Farooqi, Amjad Khan, Muhammad Luqman Sohail (2018). Molecular occurrence of canine babesiosis in rural dog population in Pakistan. Tropical Biomedicine 35(3): 1–11.

56. Shah S, A. H., Ahmad N, Akhtar R, Shehzad W, Islam S (2018). "First report on molecular detection of Toxocara species of dogs in Pakistan. The Journal of Advances in Parasitology 5(2): 25-28.

57. Maryam Javed, Farwa Saghir, Naveera Aziz, Maham Saeed, Asif Nadeem and Wasim Shehzad (2018). Genetic Screening of COMT Gene for Aggression in Domestic Bovines of Pakistan. Pakistan J. Zool., vol. 50(5), pp 1971-1973,

58. Muhammad Awais Salim, Raheela Akhtar, Muhammad Lateef, Muhammad Imran Rashid, Haroon Akbar, Wasim Shehzad, Fareeha Akhtar, Saher Islam, Shahid Hussain Farooqi, Uzma Farid Durrani 2018. First report on optimization of loop-mediated isothermal amplification (LAMP) for the diagnosis of Babesia felis. Indian Journal of Animal Research 52: 401-404.

59. Naeem H, Sana M, Islam S, Khan M, Riaz F, Zafar Z, Akbar H, Shehzad W, Rashid I 2018. Induction of Th1 type-oriented humoral response through intranasal immunization of mice with SAG1-Toxoplasma gondii polymeric nanospheres. Artificial Cells, Nanomedicine, and Biotechnology: 1-10. doi: 10.1080/21691401.2018.1478421

60. Abdullah S. Ahmad, Muhammad A. Saeed, Imran Rashid, Kamran Ashraf, Wasim Shehzad, Rebecca J. Traub, & Gad Baneth, Abdul Jabbar (2018). Molecular characterization of Hepatozoon canis from farm dogs in Pakistan. Parasitology Research, 117, 1131–1138. https://doi.org/10.1007/s00436-018-5790-1

61. Muhammad Rashid, Haroon Akbar, Imran Rashid, Khalid Saeed, Liaquat Ahmad, Abdullah Saghir Ahmad, Wasim Shehzad, Saher Islam, Shahid Farooqi (2018). Economic Significance of Tropical Theileriosis on a Holstein Friesian Dairy Farm in Pakistan. Journal of Parasitology 104(3), 310-312, (1 June 2018). https://doi.org/10.1645/16-179

62. Nisar M, Ahmad MuD, Mushtaq MH, Shehzad W, Hussain A, Nasar M, Nagaraja KV, Goyal SM 2018. Occurrence of Campylobacter in retail meat in Lahore, Pakistan. Acta Tropica. doi: https://doi.org/10.1016/j.actatropica.2018.04.030

63. Farkhanda Yasmin, Tahir Yaqub, Muhammad Idrees, Wasim Shahzad, Abu Saeed Hashmi, Kiran Aqil, Nadia Mukhtar, Muhammad Yasir Zahoor, Naeem Akhtar, Umar. S 2018. Cloning and expression of NS3 gene of Pakistani isolate type 2 dengue virus. Journal of Veterinary Research 62: 17-26. doi: 10.2478/jvetres-2018-0003.

64. Habibun Nabi, Muhammad Imran Rashid, Saher Islam, Amna Arshad Bajwa, Rahim Gul, Wasim Shehzad, Haroon Akbar, Nisar Ahmad, Aneela Zameer Durrani, Muhammad Waqas, Ashraf. K 2018. Prevalence of Toxoplasma gondii oocysts through Copro-PCR in cats at Pet Center (UVAS), Lahore, Pakistan. Journal of Pakistan Medical Association 68.

65. Habib M, Yaqub T, Nazir J, Shehzad W, Aziz-ul-Rahman, Sohail T, Mukhtar N, Mehboob A, Munir M, Shabbir MZ 2018. Genomic and biological characterization of Newcastle disease viruses isolated from migratory mallards (Anas platyrhynchos). Archives of Virology. doi: 10.1007/s00705-018-3840-8.

66. Javed M, Asif Nadeem, Babar M, Shehzad W, Abu Saeed Hashmi 2018. Novel polymorphism in OLR1 gene is associated with high milk fat content in river buffalo. Pakistan Journal of Agriculture Sciences 55: 403-407. doi: DOI: 10.21162/PAKJAS/18.4809

67. Gulam Abbas, Asif Nadeem, Masroor Elahi Babar, Tanveer Hussain, N. Aslam, Wasim Shehzad, Muhammad Tayyab, Javed Maryam (2017) Mitochondrial DNA Marker based Phylogenetic analysis of Pakistani Nilgai (Boselaphus tragocamelus). The Journal of Animal & Plant Sciences 27 (3), 776-789.

68. Munir Ahmed Bhinder, Muhammad Waqas Arshad, Muhammad Yasir Zahoor, Wasim Shehzad, Muhammad Tariq and M. I. Shabbir (2017). "Junctional Epidermolysis Bullosa (Non-Herlitz Type)." Journal of the College of Physicians and Surgeons Pakistan 27(5): 308-310.

69. Aqeela Ashraf, Muhammad Imran, Tahir Yaqub, Muhammad Tayyab, Wasim Shehzad, Thomson PC 2017. A novel multiplex PCR assay for simultaneous detection of nine clinically significant bacterial pathogens associated with bovine mastitis. Molecular & Cellular Probes 33: 57-64.

70. Sadia Munir, Asif Nadeem, Maryam Javed, Masroor Ellahi Babar, Tanveer Hussain, Wasim Shehzad, Rajput Zahid Iqbal and Sidra Manzoor (2017) Identification of pit1 gene variants in Sahiwal cattle of Pakistan. Pakistan Journal of Zoology 49 (4), 1315-1319.

71. Sadaqat Ijaz, Muhammad Yasir Zahoor, Muhammad Imran, Khushnooda Ramzan, Munir Ahmed Bhinder, Hussain Shakeel, Muhammad Iqbal, Asim Aslam, Wasim Shehzad, Huma Arshad Cheema, Habib ur Rehman (2017). Genetic analyses of fructose 1,6-biphosphotase (FB Pase) deficiencies in nine consanguineous Pakistani families Journal of Pediatrics Endocrinology & Metabolism. doi: doi.org./10.1515/jpem-2017-0188

72. Sidra Manzoor, Asif Nadeem, Masroor Ellahi Babar, Wasim Shehzad, Abu Saeed Hashmi, Muhammad Imran, Tanveer Hussain, Abdul Wajid and Maryam Javed (2017). Molecular effect of Polymorphism in the 3’UTR of Osteopontin Gene in Riverine Buffalo. Pakistan Journal of Zoology 49 (4), 1-3.

73. Madeeha Tariq, Muhammad Nawaz, Aftab Ahmad Anjum, Saba Sana, Mian Abdul Hafeez, Jawad Nazir, Wasim Shahzad, Najeeb MI 2017. Production and characterization of phytase from indigenous Aspergillus niger isolates. Pakistan Journal of Agriculture Sciences 54: 799-806.

74. Habibun Nabi, Saher Islam, Amna Arshad Bajwa, Imran Rashid1, Haroon Akbar, Wasim Shehzad, Kamran Ashraf, Nisar Ahmad, Durrani Aneela 2017. Sequence Analysis of SAG2 of Feline Toxoplasma gondii Oocysts in Pakistan. Pakistan Journal of Zoology 49: 2067-2077. doi: http://dx.doi.org/10.17582/journal.pjz/2017.49.6.2067.2077

75. Rashid Imran, Akbar Haroon, Gharbi Mohamed, Riaz Farooq, Islam Saher, Saleem Muhammad Baber, Shahzad Sammuel, Shehzad Wasim, Rouatbi Mariem, Ashraf Kamran 2017. First report of Trypanosoma evansi infection (Surra) in a Puma (Felis concolor) of Lahore Zoo, Pakistan. Journal of Zoo and Wildlife Medicine 48: 918-921. doi: 10.1638/2016-0210.1

76. Abida Rehman, Raheela Akhtar, Haroon Akbar, Farooq Riaz, Imran Rashid, Wasim Shehzad, Saher Islam, Amna Arshad Bajwa, Waqas M 2017. First report of the molecular detection of Ancylostoma caninum in Lahore, Pakistan: the threat from pets. Veterinarni Medicina 62. doi: 10.17221/14/2017-VETMED

77. Ghulam Abbas, Asif Nadeem, Masroor Ellahi Babar, Tanveer Hussain, Muhammad Sajid Tahir, Wasim Shehzad, Rajput Zahid Iqbal, Muhammad Tayyab, Maryam Javed. 2017. Molecular Phylogeny and Diversity Analysis of Hog Deer (Axis porcinus) in Pakistan. Pakistan Journal of Zoology 49: 1701-1712. DOI:http://dx.doi.org/10.17582/journal.pjz/2017.49.5.1701.1712. 

78. Muhammad Nisar, Mansur ud Din Ahmad, Muhammad Hassan Mushtaq, Wasim Shehzad, Abid Hussain, Javed Muhammad, Kakambi V. Nagaraja, Sagar M. Goyal (2017) Prevalence and antimicrobial resistance patterns of Campylobacter spp. isolated from retail meat in Lahore, Pakistan. Food Control 80 (2017) 327e332. 

79. Aslam N, Nadeem A, Babar ME, Pervez MT, Aslam M, Naveed N, Hussain T, Shehzad W, Wasim M, Bao Z, Javed M (2016) The accuracy of protein structure alignment servers. Electronic Journal of Biotechnology 20, 9-13.

80. Aslam N, Nadeem A, Babar ME, Pervez MT, Javed M, Aslam M, Wasim M, Shehzad W (2016) RPDB: A Relational Databank of Protein Structures. Pakistan Journal of Agriculture Sciences 53, 129-134.

81. Nabi H, Rashid I, Ahmad N, Durrani A, Akbar H, Islam S, Bajwa AA, Shehzad W, Ashraf K, Imran N (2016) Induction of specific humoral immune response in mice immunized with ROP18 nanospheres from Toxoplasma gondii. Parasitology Research, 1-12. DOI. 10.1007/s00436-016-5298-5

82. Sohail A, Nadeem A, Babar ME, Hussain T, Ali A, Shehzad W, Javed M (2016) β(1,4)-Galactosyltransferase-I gene polymorphisms in Pakistani Nili Ravi Buffalo. Buffalo Bulletin 35, 399-403.

83. Akhtar Ali, Masroor Ellahi Babar, Ali Raza Awan, Muhammad Tayyab, Wasim Shehzad, Muhammad Imran (2016). A Genetic Variant (S4338N) in Apolipoprotein-B Gene in Hypercholesterolemic Families from Pakistan. Pakistan Journal of Zoology 48, 1423-1429.

84. Akhtar Ali, Masroor Ellahi Babar, Ali Raza Awan, Muhammad Tayyab, Wasim Shehzad (2016) Apolipoprotein-E Gene Isoforms Genetic Spectrum in Pakistani Survivors of Myocardial Infarction. Pakistan Journal of Zoology 48, 1945-1950.

85. Imran Altaf, Anika Khalid, Jawad Nazir, Wasim Shehzad, Bashir R (2016) Outer membrane proteins and lipopolysaccharides mediated antibody response against bovine Pasteurella multocida type B-2. Malaysian Journal of Microbiology 15, 370-375.

86. Farooq Riaz, Imran Rashid, Haroon Akbar, Wasim Shehzad, Saher Islam, Amna Arshad Bajwa, Khalid Saeed, Ashraf K (2016) DNA amplification techniques for the detection of Toxoplasma gondii tissue cysts in meat producing animals. Iranian Journal of Parasitology 11, 431-440.

87. Muhammad Yasir Zahoor, M. Imran, Zara Zaheer, T Mahmood, Imran Rashid, Kamran Ashraf, Mumahhad Iqbal, Wasim Shehzad (2016), mitochondrial DNA d-loop sequence based phylogeny of Pakistani buffalo (bubalus bubalis). The Journal of Animal and Plant Sciences 26, 1886-1889.

88. Ghulam Abbas, Asif Nadeem, Masroor Ellahi Babar, Tanveer Hussain, Muhammad Sajid Tahir, Wasim Shehzad, Naeem Aslam, Muhammad Tayyab, Javed M (2016) Sequence diversity and phylogenetic analysis in Pakistani spotted deer (axis axis). Pakistan Journal of Agriculture Sciences 53.

89. Muhammad Umair Khan, Saher Islam, Amna Arshad Bajwa, Imran Rashid, Raheela Akhtar, Haroon Akbar, Wasim Shehzad, Lateef M (2016) Optimization of single step multiplex PCR for detection of Eimeria tenella and Eimeria necatrix from commercial broilers. Indian Journal of Animal Sciences 86 671–672.

90. M. A. Bhinder, M. Iqbal, M. Shahbaz, M. Y. Zahoor, Shehzad W (2015) Avian biodiversity of Bajwat wetland, District Sialkot, Pakistan. The Journal of Animal & Plant Sciences 25, 416-422.

91. Shehzad W, Nawaz MA, Pompanon F, Coissac E, Riaz T, Shah SA, Taberlet P (2015) Forest without prey: livestock sustain a leopard Panthera pardus population in Pakistan. Oryx 49, 248-253.

92. K. J. Iqbal, M. Ashraf, A. Javid, N. A. Qureshi, N. Khan, H. Azmat, M. Altaf, H. Majeed, M. S. Haider, Irfan, Shehzad W, Zahoor MY (2015) Effect of different feed ingredients on growth, sensory attributes and body composition of Labeo rohita. The Journal of Animal & Plant Sciences 25, 514-518.

93. Ijaz S, Zahoor MY, Imran M, Afzal S, Bhinder MA, Ullah I, Cheema HA, Khushnooda Ramzan K, Shehzad W (2015) Direct sequencing of FAH gene in Pakistani tyrosinemia type 1 families reveals a novel mutation. Journal of Pediatric Endocrinology & Metabolism DOI 10.1515/jpem-2015-0289.
  1. Shehzad W, Nawaz MA, Pompanon F, Coissac E, Riaz T, Shah SA, Taberlet P 2015. Forest without prey: livestock sustain a leopard Panthera pardus population in Pakistan. Oryx 49, 248-253.
  2.  Bajwa A, Islam S, Rashid I, Ashraf K, Akbar H & Shehzad W 2014. Toxoplasmosis a global threat to all vertebrates: trends in diagnostic methods. The Journal of Animal and Plant Sciences.
  3.  Fatima A, Maqbool A, Rashid MI, Akbar H, Shehzad W 2014. Haemoproteus in wild and domestic birds. Science International 26: 1.
  4.  Nabi H, Saeed K, Shah SR, Rashid MI, Haroon A, Shehzad W 2014. Epidemiological study of Gastrointestinal Nematodes of goats in District Swat, Khyber Pakhtunkhwa, Pakistan. Science International 26: 1.
  5.  Akbar H, Rashid MI, Shehzad W 2014. Parasitic Challenges to Booming Dairy Industry of Pakistan. Science International 26: 1.
  6.  Mehmood S, Lateef M, Omer MO, Anjum AA, Rashid I, Shehzad W 2014. Adulticidal and Larvicidal activity of Cassia Fistula and Piper Nigrumagainstmalaria vector. Science International 26, 775-778.
  7.  Anwar S, Lateef M, Maqbool A, Anjum AA, Rashid IM, Shehzad W 2014. Specificity of different diagnostic techniques for Ovine Haemonchosis through coprological examination. Science International 26, 249-251.
  8.  Ait Baamrane MA, Shehzad W, Ouhammou A, Abbad A, Naimi M, Coissac E, Taberlet P, Znari M 2012. Assessment of the Food Habits of the Moroccan Dorcas Gazelle in M‚ÄôSabih Talaa, West Central Morocco, Using the trnL Approach. PLoS ONE, 7, e35643.
  9.  Shehzad W, McCarthy TM, Pompanon F, Purevjav L, Coissac E, Riaz T, Taberlet P 2012. Prey Preference of Snow Leopard (Panthera uncia) in South Gobi, Mongolia.PLoS ONE, 7, e32104.
  10.  Rashid I, Akbar H, Shehzad W, Ashraf K, Ahmad N, Lateef M, Maqbool A, Oneeb M, Saeed K 2012. Prophylactic efficacies of the locally prepared Eimeria tenella vaccine in broiler chicken. Journal of Veterinary & Animal Sciences, 2
  11.  Shehzad W, Riaz T, Nawaz MA, Miquel C, Poillot C, Shah SA, Pompanon F, Coissac E, Taberlet P 2012. Carnivore diet analysis based next génération sequencing: application to the léopard cat (Prionailurus bengalensis) in Pakistan.Molecular Ecology, 21, 1951-1965.
  12.  Riaz T, Shehzad W, Viari A, Pompanon F, Taberlet P, Coissac E 2011. ecoPrimers: inference of new DNA barcode markers from whole genome sequence analysis. Nucleic Acids Research, 39, 11
  13.  Taberlet P, Prud'homme SM, Campione E, Roy J, Miquel C, Shehzad W, Gielly L, Rioux D, Choler P, Clement JC, Melodelima C, Pompanon F, Coissac E 2012. Soil sampling and isolation of extracellular DNA from large amount of starting material suitable for metabarcoding studies. Molecular Ecology, 21, 1816-1820.
  14.  Ficetola G, Coissac E, Zundel S, Riaz T, Shehzad W, Bessière J, Taberlet P, Pompanon F 2010. An In silico approach for the evaluation of DNA barcodes. BMC Genomics 11: 434.
  15.  Rashid MI, Akbar H, Al-Araby MA, Ismael AB, Shehzad W, Ashraf K, Hashmi HA 2009. Comparative efficacy of different toltrazuril doses against coccidiosis in broiler chickens. Mansoura, Veterinary Medicine Journal 11: 173 – 184.
  16.  Shehzad W, Hashmi HA, Ashraf K, Aslam A 2002. Haemoparasites of equines and their effect on blood Parameter during winter season. Pakistan Journal of Science 54:3-4.

 

 

  1. Coissac E., Riaz T., Pompanon, F., Viari A., Shehzad, W. and Taberlet, P.
  2. Evaluation and design of new DNA barcode markers for environmental studies. (Adelaide, Australia from 28 November - 3 December 2011 for the Fourth International Barcode of Life Conference).
  3. Wasim Shehzad, Christian Miquel, Eric Coissac, François Pompanon, Muhammad Ali Nawaz, Pierre Taberlet. Carnivores diet analyses using DNA barcoding. VIth European Congress of Mammalogy. Held in Paris (19-23July 2011).
  4. Wasim Shehzad, Christian Miquel, Carole Poillot, François Pompanon, Eric Coissac, Pierre Taberlet. DNA-based diet analyses in carnivores. 33ème Réunion annuelle du Groupe d’Etude de Biologie et Génétique des Populations (29 au 31 August 2011) at Muséum d’Histoire Naturelle de Toulouse, France.