Department of Microbiology

Introduction

Microbes are driving many aspects of life on the planet. The solution to many of the pressing challenges at global level, such as antimicrobial resistance; emergence of new infectious diseases; and the health of our planet’s ecosystems, rely upon broadening the horizon of discoveries coming from basic microbiology research and exploration.  We at department of microbiology are committed to provide our undergraduate students with excellent quality of Education in our degree programs of BS “Applied Microbiology” and “Medical Lab Technologists”
Our research faculty are highly productive and impactful, consisting of 5 Professors, 3 Associate and 9 Assistant Professors, housed at department of microbiology, UDL and WTO-Quality Operations Labs. All the faculty members are outstanding microbiologists who are making exciting discoveries in the field of microbiology while training students in cutting edge research. The department has  also made significant contributions in the field of conventional diagnostics and vaccines as is evident from the fact that many students both at Ph.D. and Master level had been trained and are serving in various capacities at different private and public establishments of the country. The Department is actively engaged in teaching under- and postgraduate, deficiency and diploma courses. Training workshops for field veterinarians are also being arranged. The Department has produced quite a number of M. Phil. and Ph.D. students in the University. The Department is equipped with modern tools of Miocrobiology, Molecular biology, Cell culture and the lab-animal facilities.

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Staff Position

Chairman

Professors

  1. Prof. Dr. Masood Rabbani
  2. Prof. Dr. Tahir Yaqub
  3. Prof. Dr. Aftab Ahmad Anjum

Associate Professor

  1. Dr. Muhammad Nawaz

Assistant Professors

  1. Mr. Shahan Azeem
  2. Dr. Sohail Raza
  3. Dr. Noreen Sarwar
  4. Dr. Fatima Ahsan
  5. Dr. Muhammad Asad Ali

Lecturers

  1. Mr. Imran Najeeb
  2. Mr. Hassaan Bin Aslam [Study leave]
  3. Ms. Noor-ul-Hudda [Study Leave]
  4. Mr. Muhammad Ilyas Riaz
  5. Ms. Saba Sana

Teaching Assistant 

  1. Ms. Ateeqa Aslam

HEC Approved Supervisors

  1. Prof. Dr. Masood Rabbani
  2. Prof. Dr. Tahir Yaqub
  3. Prof. Dr. Aftab Ahmad Anjum
  4. Dr. M. Nawaz
  5. Dr. Sohail Raza
  6. Dr. Noreen Sarwar

Facilities

Principal Equipment

Salient Features/Services

  • Fluorescent Spectroscope
  • Spectrophotometer
  • Refrigerated Centrifuge
  • Fluorescent Microscope
  • Inverted Microscope
  • Class I and II Safety cabinet
  • Autoclave
  • Hot air oven
  •  
  • -70 C Freezer
  • -20 C Freezer
  • Water distillation plant
  • Vertical Electrophoresis
  • Horizontal Electrophoresis
  • Sterilization
  • Sterilization
  • High speed centrifugation at low temperature
  • Fluorescent antibody technique
  • Analysis of live cells/ cell culture
  • Biochemical analysis of samples
  • To provide sterile area for lab work
  • Storage of samples & long preservation of cultures
  • Storage of samples and short preservation of cultures
  • To provide triple distilled water for cell culture & routine lab work
  • To run protein samples
  • To run DNA samples
  • Laboratory animals: Balb C/J mice colony is reared for monoclonal antibody production

Independent Labs

  • Virology
  • Bacteriology
  • Molecular Biology
  • Genome Extraction
  • Mycology
  • Cell culture
  • Influenza

Research Projects

Department of Microbiology holds long term history in research and academic activities. Its conviction for high level of research and academics is evident from the No. of PhD students and the publications in the juornals of international standards. Researchers/teachers conduct research to solve problems of the industries such as poultry, livestock, bioceutics, pharmaceutic, fish, equine and ordinary dairy farmers, milk processing, exporters and importers and government issues related to livestock. In universities every postgraduate research scholar is supposed to solve one problem of the industry/farmers. To minimize the financial consternation of the students, every Ph.D. faculty every year won at least one research grant on competitive basis from funding agencies of the country. On similar basis, following grants were obtained and postgraduate scholars conducted research on the projects and completed their research as required for their postgraduate degree program.

Research projects in progress

Title

Principal Investigator

Funding Agency

Worth (Million)

Isolation and characterization of bovine herpes virus 1 (BoHV-1) isolates from Pakistan and their use in production of gene deleted marker vaccine

Prof. Dr. Masood Rabbani

HEC, Pakistan

11.56

Analyzing the spread of anthrax in Pakistan and Jordan

Prof. Dr. Tahir Yaqub

DTRA, USA

70.701

Reducing the Economic and Zoonotic Impact of Avian Influenza (REZIAI): delivering novel vaccines and diagnostics from laboratory to the field

Prof. Dr. Tahir Yaqub

BBSRC, UK

11.324

Preparation of effective vaccine against Newcastle disease using Recombinant DNA technology and study their efficacy in chickens

Prof. Dr. Tahir Yaqub

PARB

15.120

Systematic Avian Influenza Virus Surveillance in Live Bird Markets in Pakistan

Prof. Dr. Tahir Yaqub

PSF & NSFC

10.88

Purification and standardization of mycotoxins extracted from indigenous toxigenic fungi under optimized experimental conditions

Prof. Dr. Aftab Ahmad Anjum

HEC, Pakistan

3.932

Preparation and evaluation of multivalent vaccine for Clostridium perfringens using locally characterized toxinotypes

Prof. Dr. Aftab Ahmad Anjum

HEC, Pakistan

7.902

Preparation of highly specific ELISA Kit on Recombinant outer membrane protein (OMP) of Pasteurella multocida B2 to detect Post-vaccination immune response

Prof. Dr. Aamir Ghafoor Bajwa

HEC, Pakistan

9.544

Preparation of live genetically engineered avirulent vaccinal strains derived from local highly pathogenic isolates of P. multocida B:2, using gene deletion method and evaluation of the vaccine by inoculating in mice and calves

Prof. Dr. Aamir Ghafoor Bajwa

HEC, Pakistan

6.714

Vaccine Efficacy Testing

Prof. Dr. Aamir Ghafoor Bajwa

ICI Pakistan

1.3

Hydrogen peroxide based product CareOx35 effect on the health of dairy cows

Prof. Dr. Aamir Ghafoor Bajwa

Dexon (Pvt), Pakistan

0.9

Biocontrol of Brucella abortus using brucellaphages

Dr. Ali Ahmad Sheikh

HEC, Pakistan

3.54

Development of bacteriophage based remedy for controlling Salmonella infection in chickens

Dr. Ali Ahmad Sheikh

PARB, Pakistan

13.083

Development and Evaluation of Probiotics and plant extracts targeting increased phosphorus availability and decreased ammonia emission in poultry

Dr. Muhammad Nawaz

HEC, Pakistan

3.0

Characterization, development and evaluation of indigenous probiotics targeting control and mitigation of salmonella in poultry

Dr. Muhammad Nawaz

HEC, Pakistan

6.0

Molecular characterization of bovine pestivirus isolated from dairy animals of Pakistan and their use in diagnostic, biosecurity and biological development for disease control in Pakistani herds

Dr. Arfan Ahmad

HEC, Pakistan

6.229

The development of highly sensitive novel ELISA kit for quantitative detection of 146s fraction of FMD virus for the quality production and evaluation of FMD vaccine in Pakistan

Dr. Imran Altaf

HEC, Pakistan

6.709

Evaluation of anti-Campylobacter activity of indigenous probiotic lactobacilli alone and in combination with different organic acids in poultry

Dr. Muhammad Asad Ali

HEC, Pakistan

3.429

Research projects completed

S.#

TITLE OF THE PROJECT

COST Rs. (million)

STATUS

1

Isolation and molecular characterization of Shiga Toxin producing E. Coli O157

2.316

2011

2

Augmentation of production potential of FMD+HS vaccine and immunoprophylaxis of the diseases in cattle and buffaloes

23.55

2011

3

Production and evaluation of trivalent inactivated avian influenza vaccine

2.600

2010

4

Quality testing of poultry meat and eggs

1.700

2010

5

Identification of foot and mouth disease virus from clinically infected animals and genotyping of VP 1 gene of FMD type “O” viruses

1.400

2009

6

Monitoring of chemical adulterants and hygienic status of market milk

0.700

2009

7

Sustainability of Quality Operations Laboratory, UVAS, Lahore

51.55

2009

8

Strengthening of University Diagnostic Laboratory at University of Veterinary and Animal Sciences, Lahore and animal house at Bhoneiki Campus, of UVAS, Lahore (Proposal Submitted to HEC)

39.6

2008

9

Development of protocols for production and evaluation of hydropericardium syndrome vaccine and immunised yolk/serum

0.0705

2008

10

Quality Control Laboratory for Testing Livestock and Food of Animal Origin with Reference to World Trade Organization (WTO)

99.998

2007

11

Immunoprophylaxis of Foot and Mouth Disease in bovines (ALP/PARC funded)   

5.980        

2006

12

Development of Enzyme Linked Immuno Sorbant Assay (ELISA) Kit for diagnosis of bovine infectious diseases. PCST approved

2.094

2006

13

Immunoprophylaxis of Haemorrhagic Septicaemia in bovine (University research promotion fund)

0.078

2005

14

Passive immunization of hydropericardium syndrome infected broilers (University research promotion fund)    

0.028

2004

15

Prevalence of egg drop syndrome virus in commercial poultry (University research promotion fund) 

0.055

2002

16

Immunobiology of Newcastle disease virus in poultry (University research promotion fund)

0.042   

2000

17

Preparation and evaluation of Mycoplasma capri vaccines (University research promotion fund)

0.087

2000

18

Characterization of avian influenza in poultry (funded by Olympia Poultry Breeders-PVT-Ltd,  Big Birds- PVT-Ltd, Avicenna Labs -PVT-Ltd)

0.087

1997

 

Partial Ecology and Epidemiology of Soil borne Human and Animal Bacterial Pathogens and its Public Health Significance in Pakistan

72.90

2018

Human Resource Development (Workshops):

S.#

Title

Duration (Days)

Dates

1

Primer designing for microbial identification using PCR

2

09-10 November-09

2

Advanced Laboratory Techniques

12

04-16 May 2009

3

Advanced Diagnostic Techniques and Control of Poultry Diseases

6

15-20 March 2010

4

Basic diseases diagnostic techniques in Livestock and poultry

6

05-10 April 2010

5

Applied Research on advanced laboratory techniques

4

17-21 may 2010

6

Molecular techniques (PCR/RT-PCR) for diagnosis of animal microbial diseases

4

16-19 November 
2009

7

Monitoring of raw milk for sorbitol non-fermenting E. coli

5

17-21 May 2010

8

Propagation of Newcastle disease virus in chicken embryos and detection of its immunogenic protein using SDS-PAGE

5

31-May-10
07-June-10
12-July-10

9

Hygienic Monitoring of Food and Food Products

5

05-09 July 
2010

10

Isolation, Screening and Preliminary Characterization of Antimicrobial Metabolites from Food Microbes

5

19-23 July 2010

11

Preparation and Evaluation of Oil based Multivalent Foot and Mouth Disease and Hemorrhagic Septicemia  Vaccine

5

02-11 August
2010

12

Bio-fermentation for Biomass Production of Pasteurella multocida

5

23-27 August 2010

13

Diagnostic approaches towards avian Mycoplasmosis  with collaboration of USA aid project Prof. Dr. Muzaher 
Hussain Khan

2

06-07 November 2011

14

Int. Poultry Nutrition Conference and Expo-15

2

3-4, November, 2015.

15

Int. Human Nutrition Conference and Expo-15.

2

3-4, November, 2015.

 

16

37th All Pakistan Science   Conference

2

16-17, November, 2015

 

17

International  training workshop on biosafety and risk assessment 2015

2015

 

18

Basic Laboratory techniques 2015

2015

 

19

International workshop on “Emerging and Re-emerging Infectious Diseases

2

September 14-16, 2015

 

20

Consensus Building Workshop On Biorisk Management Guidelines

2015

 

21

International Training Workshop on Biosafety and Risk Assessment

2015

22

In Service Short Term Training of University and College Faculty

5

16th -20th May, 2016

23

 Molecular Diagnostics

3

23-25 June 2015

24

Basic Laboratory Techniques 
(as Resource Person)

5

03-07 Aug, 2015

 

25

Basic Laboratory Techniques 
(as Resource Person)

5

03-07 Aug, 2015

 

26

Workshop on “Advanced Lab techniques-1 “ for Lab assistants and Lab attendants of UVAS

3

July, 20-22, 2016

27

Training of young faculty members of SBBUVAS (Sakrand) for two weeks

14

July, 17-30, 2016

 

28

In-service training for university and college faculty for Faculty of Bio-Sciences, UVAS, Lahore

5

August, 1-5, 2016

29

In-service training for university and college faculty for Department of Microbiology, FVS, UVAS, Lahore

3

August, 3-5, 2016

30

Teaching methodologies and resource management of Veterinary Anatomy and Histology courses

5

October, 3-7, 2016

31

Two days training on Skills Enhancement of Teaching Staff of LSTCs/Training Institutes

2

March, 30-31, 2017

32

International workshop on “Devise strategies to control emerging viral hemorrhagic fever in Pakistan”

3

14th to 16th November, 2016

Research Priorities

  • Isolation and identification of the causative agents of the newly emerging microbial diseases
  • Preparation and evaluation of biologics (vaccines, probiotics, antimicrobials, diagnostic reagents, etc.)
  • Qualitative and quantitative detection of mycotoxins and measures to overcome mycotoxicosis in animals
  • Physico-chemical factors affecting the survival of microbial pathogens (Biosecurity)
  • Immunogenetics, Immunotherapy, Immunomodulation, Immunoprophylaxis Immunoregulations and Immunodiagnosis of various diseases
  • Studies of the Zoonotic Diseases